PJB-2023-620
MOLECULAR, PHYSIOLOGICAL AND MORPHOLOGICAL MARKERS ASSISTED SELECTION FOR SALT-TOLERANT IN SOME EGYPTIAN FABA BEAN VERITIES
MOHAMED H. ABDELFATTAH
Abstract
Salinity stress is one of the most serious abiotic factors limiting faba bean productivity. The faba bean is an important legume crop because it can give a high yield, and its grains are full of nutrients. This study aims to select by several markers the best variety that adapts to salt stress. Three varieties of Vicia faba (Nubaria-1, Nubaria-3, and Nubaria-4) were used in this study. Plant growth parameters, cell membrane stability, photosynthesis pigments and protein profile analysis were measured as indices for salinity responses. Also, this study used ten ISSR primers to assess the genetic diversity of three varieties. All determinations were carried out under control and salinity stress conditions. Our results indicated that the Nubaria-3 variety performed most effectively regarding root characteristics and the ability to develop well under salt conditions compared to the other varieties. Changes in protein profile increased in band number and intensity, and its expression continued under salinity conditions; this appeared with Nubaria-3 and Nubaria-4. Polymorphism and phylogenetic relationship among varieties were investigated by ISSR molecular markers and detected 22.43% polymorphism, and the highest percentage of genetic similarity between Nubaria-3 and Nubaria-4 was 73%. Our data conclude that the Nubaria-3 variety performed most effectively regarding root characteristics and the ability to develop well under salt conditions compared to the other varieties. As a result, it was chosen as a tolerant variety in a subsequent investigation to explore the most essential genes associated with salt tolerance compared to the sensitive variety.
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