PJB-2023-560
GCMS ANALYSIS AND BIOLOGICAL ACTIVITIES OF PROPOLIS OIL FROM NORTH PUNJAB, PAKISTAN.
SHABANA ISLAM
Abstract
Propolis, a resinous material prepared by honey bees from exudates and floral sources, have extensive range of biological properties and important part of herbal medicinal system. The research work is the first report to investigate the chemical composition and biological potential of the oil extracted from brown propolis sourced from Northern Punjab, Pakistan. The extraction of oil has been carried out by microwave affording (0.615%). The phytochemical constituents (45 compounds) were identified by gas chromatography-mass spectrometry (GC-MS), where, sesquiterpenoid (α-eudesmol and (-)-β-Copaene) while sesquiterpenes including τ-cadinol, Calamenene andα-Humulene) were the most prominent natural products. The results showed two unknown compounds (5%) which remain unidentified due to unavailability of data on NIST-2011. The volatile oil derived from brown propolis shown remarkable efficacy in terms of its antioxidant (IC50 0.54 ± 0.07mg/mL), anti-inflammatory (IC50 51.0239± 0.01mg/mL), antitumor (against the HeLa-human cervical cancer cell line) (IC50 1.96± 1.52 mg/mL), antifungal (A. niger (MIC > 3mg/mL), A. flavus (MIC > 5mg/mL) and antibacterial (Bs: Bacillus mojavensis (MIC 0.421mg/mL), Sa: Staphylococcus aureus (MIC 0.501mg/mL), Pa: Pseudomonas aeruginosa (MIC 0.829mg/mL), Ec: Escherichia coli (MIC 0.943mg/mL) and St: Salmonella typhimurium (MIC > 2mg/mL). Therefore, propolis oil has potential therapeutic agents and can be applied as readily available source for treating microbial infections.
Key word: Propolis oil, GC-MS, antibacterial, anti-inflammatory, antitumor activities
To Cite this article:
Download
