PJB-2023-556
IN VITRO PROPAGATION OF PAULOWNIA TOMENTOSA L. BY USING METHYL JASMONATE; A POTENT PLANT GROWTH REGULATOR
Aisha Iqbal
Abstract
Paulownia tomentosa is an economically important fast growing timber tree belongs to family Scrophulariaceae. Because of slow germination rate, this plant requires relatively long time to propagate through seeds. Thus, present investigation was aimed at finding an efficient in vitro method for multiplying this valuable plant. Nodal plant parts were taken as explants source from one-year healthy plant. For callus induction and regeneration explants was inoculated on MS medium with various concentrations of NAA and methyl jasmonate (MeJ) for shoot production. Results showed that methyl jasmonate (60 µM) and NAA (0.5mg/L) in combination-initiated shoots after 19 days with the highest shoot length of 10 cm and maximum number of leaves and nodes which were 14 and 13, respectively. Shoots produced under in vitro conditions were cultured on root induction medium (MS half strength) with different IBA concentrations (0 - 2.5 mg/L). MS medium alone without IBA treatment was not shown any rooting response. However, MS (half strength) medium with IBA (2.5 mg/L) concentration produced roots after 13 days of inoculation and 5 roots were formed per shoot.
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