PJB-2023-541
PHENOLIC COMPOSITION, ANTIOXIDANT ACTIVITY AND ANTIMICROBIAL ATTRIBUTES OF EREMOSTACHYS VICARYI BENTH.EX HOOK.F. FROM SOON VALLEY, SALT RANGE, PAKISTAN
Amin Shah
Abstract
The research work was carried out to study the phenolic composition, antioxidant activity and antimicrobial activities of Eremostachys vicaryi Benth. ex Hook.f. by using aqueous and ethanol extracts. The flavonoid content of the extracts varied significantly due to the utilization of two distinct extraction approaches, namely sun-drying and shade-drying. The ethanol extracts obtained from sun-dried samples had a significantly greater flavonoid content of 634.568±2.14 mg/100g CE, whereas the ethanol extracts from shade-dried samples had a lesser content of 253.926±35.30 mg/100g CE. The aqueous extracts obtained from samples that were dried in the sun and in the shade showed flavonoid content values of 148.148±3.70 mg/100g CE and 188.889±3.70 mg/100g CE respectively. The aqueous shade-dried extracts had the highest recorded phenolic content of 1554.00±1.00 mg/100g GAE, while the ethanol sun-dried extracts had the least recorded phenolic content of 401.00±1.00 mg/100g GAE. The antioxidant activity exhibited variation, with the maximum recorded value of 2776.22±2.70 µg/100g AAE reported in aqueous sun-dried extracts and the lowest recorded value of 2093.33±5.63 µg/100g AAE in aqueous shade-dried extracts. Both polar and non-polar extracts were used on four bacterial strains (2-gram positive and 2 gram negative) and four fungal strains to determine antimicrobial activity. Maximum antibacterial activity was observed in chloroform extracts, zone of inhibition (ZI) valued at 26±1 mm against Staphylococcus aureus and minimum was observed in aqueous extracts, ZI 8.57 mm against Psedomonas aeruginosa. ZI against fungal strains was maximum for methanol extracts 24±2.51 mm against Aspergillus niger and minimum was for petroleum ether extracts ZI 8±1.52mm against Aspergillus oryzae.
Keywords: Eremostachys vicaryi, Soon Valley, Antibacterial, Folin-Ciocalteu reagent, toxic plants
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