PJB-2023-482
MYC2 negatively regulate erucic acid synthesis in Brassica napus
xi jiang
Abstract
Erucic acid is a type of long-chain fatty acid in liquid synthesis. Many double low rapeseed varieties have been selected because long-term consumption of rapeseed oil with high erucic acid content is considered to harmful health. MYC2 is the crucial regulator in the JA signaling pathway, which participates in regulatory plant response to insect, pathogenic bacteria and other stress. However, whether MYC2 involves in other biological processes is unclear. In this study, we reported that BnMYC2 negatively modulates erucic acid synthesis by directly binding promoter of BnFAE1, which is the key gene during the erucic acid synthesis in Brassica napus. GUS histochemistry assay showed that staining was observed in all detected tissues, especially in seed. The subcellular localization indicated that the protein encoded by the gene was distributed in the nucleus. Yeast one-hybridization and double luciferase experiments exhibited that BnFAE1 expression was regulated by BnMYC2 in vitro and in vivo, respectively. The results of genetic transformation verified that compared with WT, the expression level of AtFAE1 gene and the erucic acid content were significantly enhanced in the mutant myc2-2. Unexpectedly, the expression level of AtFAE1 gene and the erucic acid content were significantly declined in overexpression transgenic Arabidopsis seeds. Taken together, these results revealed that BnMYC2 is played a significant role in the process of erucic acid synthesis in oilseed rape. Our research provided a preliminary understanding of how erucic acid synthesis was mediated by JA signaling in Brassica napus
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