PJB-2024-1512
Modulation of leaf senescence in Bergenia ciliata (Haw.) Sternb. through the supplementation of kinetin and Methyl jasmonate
Foziya Altaf, Shazia Parveen, Mohammad Lateef Lone, Aehsan Ul Haq, Sumira Farooq, Inayatullah Tahir, Sheikh Mansoor and Mohammed Nasser Alyemeni
Abstract
Optimizing senescence in specific plant species is a significant objective in crop breeding programs. Senescence in plants serves to redistribute nutrients from older tissues to new organs at the end of the growing season, contributing to parental investment for improved growth fitness. This study aimed to investigate the potential role of exogenous kinetin (KIN) and methyl jasmonate (MeJ) in regulating postharvest leaf senescence in Bergenia ciliata at two stages of leaf development (referred to as S1 and S2). Various physiological and biochemical parameters were evaluated to understand hormonal control and crosstalk during senescence, considering the integration of developmental signals. Leaf discs were prepared from excised leaves of B. ciliata and treated with different concentrations of KIN and MeJ (0.01, 0.1, 1 and 10 µM), alongside a control set. Compared to the control, KIN application extended the green color of leaf discs by approximately 4 days, delaying leaf senescence. This delayed senescence was accompanied by higher levels of chlorophyll and soluble proteins, along with a noticeable decrease in sugar levels. Moreover, leaf discs treated with various KIN concentrations exhibited a significant increase in antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), while lipoxygenase (LOX) activity decreased significantly. On the other hand, leaf discs supplemented with different concentrations of MeJ showed discoloration, lower chlorophyll content, reduced soluble proteins and increased sugar levels compared to the control. However, MeJ application enhanced the activity of antioxidant enzymes in the leaf discs, demonstrating a synergistic effect with KIN in augmenting antioxidant enzyme activity. By investigating these parameters, the researchers aim to elucidate how KIN and MeJ modulate postharvest leaf senescence in B. ciliata by analyzing underlying physiological and biochemical mechanisms involved
To Cite this article:
Altaf, F., S. Parveen, M.L. Lone, A.U. Haq, S. Farooq, I. Tahir, S. Mansoor and M.N. Alyemeni. 2024. Modulation of leaf senescence in Bergenia ciliata (Haw.) Sternb. through the supplementation of kinetin and Methyl jasmonate. Pak. J. Bot., 56(2): DOI: http://dx.doi.org/10.30848/PJB2024-2(6)
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