PJB-2023-472
Phytochemical Screening, In Vitro Antimicrobial and Cytotoxic Potential of Medicinally Important Plantago Amplexicaulis. L
Muhammad Adil
Abstract
Medicinal plants are considered as the most important source for the development and discovery of natural drugs. The present research work was carried out to investigate the phytochemical constituents, antimicrobial activity and cytotoxic activity of the methanolic and ether extracts of Plantago amplexicaulis L. The phytochemical screening revealed that the ether extract was rich in terpenoids, flavonoids, tannins, proteins, and saponins whereas the methanolic extract of P. amplexicaulis was rich in terpenoids, flavonoids, phytosterols, tannins, proteins, phenols, and saponins. The antibacterial activity of methanolic extract of P. amplexicaulis had maximum inhibitory zone of 29 mm against Klebsiella pneumonia with 30 mg/mL concentration and the minimum inhibitory zone was recorded as 13 mm at concentration of 10mg/mL against Staphylococcus aureus with maximum MIC value of 8mm against Escherichia coli. The ether extract of P. amplexicaulis had maximum inhibitory zone of 27 mm against Klebsiella pneumonia with 30 mg/ml of extract while the minimum zone of inhibition was 12 mm against Shigella flexneri at 10 mg/ml concentration with maximum MIC value of 8mm against S. flexneri. 30 mg/ml of methanolic extract in antifungal activity showed maximum zone of inhibition of 25 mm against Aspergillus fumigatus and the minimum zone of inhibition was 8 mm against Fusarium solani at concentration of 10 mg/ml with maximum MIC value of 8 mm against F. solani. 30 mg/ml of ether extract showed maximum zone of inhibition of 24 mm against F. solani while the minimum inhibition zone was 7 mm against A. fumigatus at concentration of 10 mg/ml with highest MIC value of 9 mm against A. fumigatus.
1000 mg/ml of methanol extract showed high mortality rate (293.33%) of brine shrimps while shown lowest (26.66) at 10 mg/ml. Similarly, 10 mg/ml of ether extract revealed high mortality rate (253.33%). The plant was found a potent therapeutic agent against microbial infections and highly cytotoxic.
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