PJB-2023-455
Rahat Naseer
Abstract
Mycotoxins are the major causes of diseases and deaths both in humans and animals and detoxification of these mycotoxins were performed using leaves extracts. The methanolic extracts from leaves of Azadirachta indica were prepared using 80% aqueous methanol at 37 °C and 150 rpm. A. indica leave extracts were estimated for the presence of antifungal compound such as terpenoids (50mg/25g). The detoxification potential was evaluated by Fungus Inhibition Assay (FIA) using incubation with purified toxins of Aspergillus flavus and Aspergillus ochraceous. The maximum inhibitions among different neem leaves methanolic extracts (10%, 15%, 20%) were performed while 20% extract gave 69-84% inhibition against Aspergillus flavus and 64-83% against Aspergillus ochraceous. Toxicity of mycotoxins at biological level was also checked at cell cycle stage with corn germinating seeds. Methanolic extracts of Azadirachta indica showed significant inhibition of mold growth. This research activity will boost the antifungal activity of Azadirachta indica leave extract against mycotoxins of fungi and proves vital for pharmaceutical industry.
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