PJB-2023-431
EFFECT OF FOLIAR APPLICATION AND SEED PRIMING OF CHITOSAN ON RADISH UNDER DROUGHT STRESS
zainab Tamoor
Abstract
Plants are facing different kinds of stresses both biotic and abiotic but water stress is one of the main stress faced by plants, now a days. Chitosan is reported to act as an anti stress substance for many crops. It has been shown to increase plant growth and development. Its different application like foliar spray and seed priming helps to reduce adverse effects of drought stress and improve water use efficiency. But no comparative research was performed in order to evaluate the effect of seed priming and foliar application of chitosan on radish under water stress. So, this research was done to study the impact of various concentration of chitosan (75ppm and 150ppm) on radish (Raphanus sativus L.) with its foliar application and seed priming. Utilizing a Randomized Complete Block Design, the experiment involved 30 pots subjected to different treatments. Ten treatments were included as : control, water stressed, chitosan seed priming at 75ppm and at 150ppm, chitosan foliar application at 75ppm and at 150ppm, chitosan seed priming with water stress at 75ppm and at 150ppm, chitosan foliar application (75ppm and at 150ppm) with water stress. Parameters including percentage of germination, seedling vigor index, root and shoot length, seedling vigor, dry weight and fresh weight, osmotic potential, membrane stability index, relative water (RWC), chlorophyll, carotenoid, sugar, proline, and protein content were assessed at vegetative stage of the plant. Chitosan applications showed noticeable improvement in growth parameters even in stress conditions. Furthermore, there was an increase of protein proline and osmotic potential in plants which were treated with chitosan. In addition, chitosan decreases the proline content which was accumulated in stress. The results highlighted the potential of chitosan to contract negative impacts of water stress on overall growth of plant and development especially at high concentration (150ppm) both as foliar application and seed priming.
To Cite this article:
Download
