PJB-2023-421
METABOLIC CHANGES IN TOMATO LEAVES INFECTED BY TOMATO YELLOW LEAF CURL VIRUS
Faiza Aslam
Abstract
Tomato yellow leaf curl virus (TYLCV) is one of the most devastating tomato-infecting begomoviruses that is transmitted by whitefly (Bemisia tabaci). In this study, a field survey was carried out to collect samples infected with TYLCV. Samples were collected on the basis of their morphological changes i.e., curling, crumping, chorosis of leaves besides stunted growth. Confirmation of TYLCV was confirmed by applying ELISA test. Diseased samples were later used as virus inoculum. After that, tomato plants were grown under shed condition. After 15 days of seeds sowing, seedlings having proper height were transferred individually into new pots and divided the plants into two groups. First contained experimental and second contained control. The viral inoculums of TYLCV were infiltrated to experimental group. Significantly enhanced tomato growth parameters that alleviated the negative morphological impacts resulting in all experimental plants compared to control plants. These treatments resulted in noteworthy changes in GCMS metabolites like amount of Glucose 6 was highest and shikimate was lowest in control plants in quantity whilst in experimental plants gluconate compound was highest in quantity and glutamic acid was present in negligible amount. Accordingly, these compounds could potentially be used as environment friendly plant growth promoters and defense modulatory agents to protect tomato plants from diseases caused by the TYLCV pathosystems.
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