PJB-2023-349
Screening of Plant Phenolics for Antimicrobial Efficacy
Ekta Menghani
Abstract
Background Plants have been used as therapeutical agents for more than 5000 years. Some of their applications and medicinal effects have already been scientifically proven, while others still need to be further researched. Our aim was to screen more than 30 different colored plant extracts for antimicrobial properties against Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus. Colored plant parts were chosen because the focus of our research was set on anthocyanins as antimicrobial agents.
Methods Therefore, alcoholic cold extracts were produced of each plant and tested against the different strains, using the agar-well diffusion method. To identify the compounds of the plants thin-layer chromatography was performed.
Results Among all the cold extracts tested, cloves and pomegranate skin showed the highest significant activity against E. coli and S. aureus. Other extracts showing growth inhibitory properties against different strains include lychee skin, jamun and lemon skin.
Antimicrobial activity against the gram-negative E. coli was proven significant (p- value < 0,001) for cloves and pomegranate skin compared to the standard streptomycin. The IZ value of pomegranate skin was measured 27.5 ± 2.5 and the IZ for cloves 27.5 ± 1.5, whereas the mean IZ of streptomycin was identified to be 13.1 ± 8.9.
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