PJB-2023-343
EVALUATION OF FUNGICIDES AND BIOPESTICIDES FOR THE CONTROL OF PHYTOPHTHORA CITROPHTHORA IN-VITRO CONDITION
YASIR IFTIKHAR
Abstract
Gummosis caused by P. citrophthora frequently occurs, leading to considerable losses in major citrus growing areas of the country. In the present study, different fungal and bacterial biocontrol agents (Trichoderma harzianum, Trichoderma atroviridae and Trichoderma viridae, Bacillus spp. and pseudomonas spp, plant extracts (Neem, Eucalyptus, Mint and garlic) and chemicals (Tebuconazole, Topsin-M, Metalaxyl + Mancozeb, Copper oxychloride, Aliette) were evaluated against P. citrophthora under in vitro conditions. T. harzianum, inhibited 90% of the mycelial growth of the pathogen, followed by T. atroviridae, who showed 81% of the percent inhibition on the 7th day of the treatment. T. viridae also significantly inhibits the pathogen up to 73% on the 5th day of the treatment but less than T. harzianum and T.atroviridae. The bacterial biocontrol agents inhibited 62.76 to 77.96% growth of P. citrophthora. In botanical control, eucalyptus gave maximum inhibition of 88%, 91%, and 91%, followed by the mint of 69%, 61%, and 61% on the 3rd, 5th and 7th days. In contrast, neem and garlic gave low percent inhibition compared to eucalyptus and mint. Garlic gave up to 31% maximum percent inhibition on the 3rd day, and neem was 49% on the 7th day. Among the chemicals, higher percent inhibition was given by Tebuconazole 78% at 300 ppm on the 7th day of the treatment, followed by Aliette 72% at 100ppm on 5th day and then Topsin-M 66% at 300ppm on 7th day of the treatment. All plant extracts, inhibited the mycelial growth of P. citrophthora at 3rd, 5th and 7th day.
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