PJB-2023-278
PHYTOCHEMICAL ANALYSIS AND BIOLOGICAL ACTIVITIES OF RUBUS ELLIPTICUS AND ELAEAGNUS UMBELLATE ROOT EXTRACTS
SAMI ULLAH KHAN
Abstract
Medicinal plants are used for remedial and health purposes in developing and advanced countries, where they serve as an important source for the cure of several diseases and infections that affect approximately 70% of the population. The current study detects and quantifies a secondary metabolite and antibacterial activities of roots extract of Rubus ellipticus and Elaeagnus umbellate. The phenolics content was determined using Folin-Ciocalteu reagent method and aluminum chloride colorimetric method with some modifications for total flavonoids estimation. The antioxidant activity was determined by DPPH free radical scavenging assay and reducing power assay. Agar Well Diffusion method was used to test the antibacterial activity of R. ellipticus and E. umbellata against two bacterial strains, Escherichia coli and Rhizobium leguminosarum. Our findings indicated that phenolic content of R. ellipticus and E. umbellate was found to be 209.31 and 161.89 µg GAE/ mg/extract, respectively. The highest flavonoid content was found in methanol extracts of R. ellipticus (150.219. µg QE/mg) and E. umbellate (133.35µg QE/mg). The reducing power activity of R .ellipticus methanol extract was found to be high as compare to E. umbellate. The present study revealed that phenolic and flavonoid contents exits in extracts of R. ellipticus and E. umbellata. Furthermore, these plant extracts have antioxidant and antibacterial properties against two bacterial strains i.e. E. coli and R. leguminosarum. Phytochemicals derived from the roots of R. ellipticus and E. umbellata may have pharmacological benefits like anticancer, anti-inflammatory, antifungal and antibacterial properties.
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