PJB-2023-247
Optimize the culture and fermentation condition of biocontrol strain pH-120 against Sclerotinia sclerotiorum
Dan Zhang, Xinyue Ma, Xilin Sun, Yufan Wang, Xi Zhao, Ronglie Gong and Xiaomei Wang
Abstract
Sclerotinia sclerotiorum is the main disease in cucumber cultivation in northern China. In the early stage of laboratory, a biocontrol strain Penicillium halotolerans (Ph-120) with high-efficiency antagonistic effect on the pathogen was screened out from cucumber rhizosphere soil. In order to improve the antifungal activity of the fermentation broth of Ph-120 strain against Sclerotinia sclerotiorum, the culture conditions and fermentation conditions of Ph-120 strain were optimized by single factor test, orthogonal test and response surface methodology. The results showed that the hyphae of Ph-120 strain were affected by different single factors in the following order: temperature > nitrogen source > carbon source > pH; The optimal fermentation conditions were as follows: carbon source amount 24.0g/L, nitrogen source amount 2.2g/L, fermentation temperature 25℃, and pH value 6. The antifungal rate of the fermentation broth against pathogen was 94.6% after optimization in model verification experiment, which was 16.7% higher than that under the optimization condition. The experiment provide a theoretical basis for that development of biopesticide
To Cite this article:
Zhang, D., X. Ma, X. Sun, Y. Wang, X. Zhao, R. Gong and X. Wang. 2024. Optimize the culture and fermentation condition of biocontrol strain pH-120 against Sclerotinia sclerotiorum. Pak. J. Bot., 56(5): DOI: http://dx.doi.org/10.30848/PJB2024-5(10)
Download
