PJB-2023-216
Evaluation and identification of antibacterial, antifungal, antioxidant, and phytotoxic activities of Asplenium dalhousiae Hook. a potential medicinal pteridophyte
Shanza Zaib, Simona Klačanová, Robina Khan, Nazish Bibi and Manzoor Hussain
Abstract
Pteridophytes have been identified as resistant to microbial infections, which may be attributed to the presence of various phytocompounds. The current study focused on the Asplenium dalhousieae Hook for detailed morphological, pharmacognostic, and phytochemical evaluation. Here we used the fresh and powdered fronds of Asplenium dalhousieae for its macroscopic and microscopic characterization. Phytochemical investigations were carried out by using ethanolic, methanolic, and distilled water extracts. Antimicrobial, phytotoxic, and antioxidant properties were assessed using crude methanolic extracts. The macroscopic characteristic of the plant revealed a dark green color, smooth powdered medicine texture, and an aromatic odor. Different types of cells and their micrometry were detected in microscopic features, confirming that the Asplenium dalhousieae has a wide range of cells such as subsidiary cells, scalariform arteries, desmocytic stomata, and polygonal epidermal cells. Phytochemical analysis showed the existence of multiple phytochemical compounds such as flavonoids, phenols, glycosides, alkaloids, coumarin, anthraquinones, quinones, cardiac glycosides, terpenoids, and saponins. Subsequently, plant antimicrobial response against various bacterial and fungal strains (Streptococcus mitis, Pseudomonas fluorescens, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae Proteus mirabilis, and Salmonella typhi and Carvularia, Fusarium, Alternaria, Rhizopus, Aspergillus) determined a significant response when compared with standard drug ciprofloxacin and miconazole respectively. The phytotoxic response against the test plant Lemna minor indicated 75% growth of inhibition revealing potent herbicidal aptitude. Further, antioxidant potential revealed 34% inhibition by DPPH and 55.7% inhibition by phosomolybdate complex assay. In conclusion, this study emphasizes the promising therapeutic applications of Asplenium dalhousieae in diverse medical contexts, highlighting its substantial potential for future research and development. Further studies on Asplenium dalhousieae are needed to identify and isolate cytotoxic chemicals, as well as to use TLC methods to aid the production of natural bioactive compounds
To Cite this article:
Zaib, S., S. Klačanová, R. Khan, N. Bibi and M. Hussain. 2024. Evaluation and identification of antibacterial, antifungal, antioxidant, and phytotoxic activities of Asplenium dalhousiae Hook. a potential medicinal pteridophyte. Pak. J. Bot., 56(5): DOI: http://dx.doi.org/10.30848/PJB2024-5(32)
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