PJB-2023-210
Assessing Molecular Diversity of Alternaria sesami using RAPD Marker
Shivam Maurya
Abstract
Sesame (Sesamum indicum) an economically important food crop is affected by fungal, bacterial and viral diseases. The most important fungal disease is Alternaria blight caused by Alternaria sesami. Alternaria blight can be controlled by using fungicides, biocontrol agents, phytoextract and cultural practices. A sustainable control measure is the development of resistant sesame cultivar. A prerequisite to this approach is the knowledge of genetic diversity of this fungal pathogen. Molecular diversity analysis was done through RAPD marker and by using 10 random primers from OPD series i.e., OPD 01-10. Primers OPD-03, OPD-04, OPD-08 and OPD-10 showed 100 per cent polymorphism and least polymorphism was observed with primer OPD-09 (95.34 %). In the similarity matrix, isolate AsPA1 was found to have a higher value of similarity coefficient (1.00) while, isolate AsSM2 was found to have a lower value of similarity coefficient (.036).
To Cite this article:
Download
