PJB-2023-186
Exploration of Antidiabetic Potential of Traditional Ethnomedicinal Plant: Viscum cruciatum Sieber ex. Spreng. from Azad Jammu and Kashmir, Pakistan
Waheeda Mushtaq
Abstract
The current research was aimed to explore antihyperglycemic potential of an ethnomedicinal plant Viscum cruciatum Sieber ex Spreng (VCSS) family Loranthaceae from Neelam valley of Azad Jammu and Kashmir (AJK), Pakistan. Ethnomedicinal survey, phytochemical profiling and analysis (EPA) model was used to study antidiabetic potential of the plant in alloxan induced rat-models. Traditional ethnomedicinal (TEM) knowledge was collected using Open-ended and close-ended questionnaire method. TEM data was authenticated using microstatistical tools: informant consensus factor (ICF) and fidelity level (FL) in which highest FL was 72.86% for plant sample of Viscum cruciatum taken from host Olea ferruginea. The highest ICF value (11.8) was found for diabetics and lowest value (ICF=1.7) for anxiety. The preliminary phytochemical screening of leaf and bark was conducted using methanol solvent which depicted presence of flavonoids, saponins and alkaloids phytoconstituents.
For antihyperglycemic analysis, experimental trials were carried out in laboratory using alloxan induced diabetic model animals Oryctolagus cuniculus L (rabbits). The antidiabetic effect of MeOH extracts of leaf and bark at conc. of 500 mg/kg and 1000 mg/kg in normal, glucose loaded diabetic and alloxan induced Type-2 hyperglycemic rabbits was assessed by oral dose therapy for 7, 15 and 30 days. The blood sugar level of normal control group (NCG), diabetic control group (DCG), treatment control group (TCG) with Glibenclamide and trial groups (TGs) was checked using ACCU-CHEK-active model glucometer (Roche Co.). The results depicted that T6-group treated with1000 mg leaf extract from host O. ferruginea showed maximum decrease in blood glucose level i.e 149±1.5 mg/dl. It was found that body weight, trial group T6-group showed maximum decrease (25.36%) in body weight (treated with 1000mg leaf extract of plant from host O. ferruginea) while less decrease (8.22%) was observed in T3-group (treated with 500mg bark extract of plant from host Querqus). For serum insulin level, T6-group showed maximum increase (5.79±0.11pmol/L) on 15th day of treatment. The study demonstrated that methanolic extract of VCSS has great potential of antihyperglycemic property which can be used to cure in Type-2 diabetes mellitus, thus justifying TEMs use of Viscum cruciatum plant from AJK territory. Through future comprehensive research novel techniques allopathic drug can be produced and optimized from this wildly occurring plant.
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