PJB-2023-168
gongyuqiao
Abstract
Plant variety and cultivar identification is one of the most important and problematic aspects in agricultural systems. This study is for barcoding of 12 Physalis Peruviana L landraces in northeast of China with three specific primers (ITS2, matK, rbcL) and their combinations loci such as rbcl+matK, ITS2+rbcl, ITS2+matK, ITS2+rbcl+matK. Otherwise, 10 SSR markers were used to assess the genetic diversity of the landraces. The phylogenetic trees of ITS2+rbcL and rbcL+matk landraces of Physalis Peruviana L in a separate clade demonstrating that the combinations loci ITS2+rbcL and rbcL+matk have sufficient efficiency for landraces discrimination of Physalis Peruviana. Moreover, the Mantel test showed that genetic distance had no significant correlations with geographic distance. AMOVA analysis showed that the main Physalis Peruviana L landraces variation of this garlic germplasm collection existed in the within-population or cluster.
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