PJB-2023-68
CHARACTERIZATION AND IN-VITRO ANTIBACTERIAL POTENTIAL OF ANTIBIOTICS AGAINST PECTOBACTERIUM ATROCEPTICUM
Nadia Liaqat
Abstract
Black leg is the most devastating threat being faced by Potato caused by Pectobacterium atrocepticum (Pa). Diseased samples were collected and after isolation, pathogen was confirmed through Koch’s postulates, gram staining, KOH test and Potato tuber assay. In the Phyto-bacteriology Laboratory at the University of Agriculture Faisalabad's Department of Plant Pathology, a trial was conducted using the inhibition zone technique under a Completely Randomized Design (CRD). Levofloxacin, Streptomycin, Ampicillin, Kanamycin, Erythromycin, Ciprofloxacin, Clarithromycin, Azithromycin, and Amoxicillin were the nine antibiotics evaluated using the inhibition zone technique in a completely randomized design against Pa. Testing was done at three concentrations (300, 500, and 700 ppm). Levofloxacin produced the best results out of these nine antibiotics with a mean inhibition zone of 14.22 mm, followed by Ciprofloxacin (13.18), Ampicillin (6.89), Kanamycin (6.64), Clarithromycin (6.01), Azithromycin (5.24), Streptomycin (4.98), Amoxicillin (4.79), and Erythromycin (4.29), in that order. Levofloxacin showed highest inhibition zone (15.00 mm) after 36 hours while Ciprofloxacin showed maximum inhibition zone (15.06 mm) at 700 ppm in the interaction between treatments and concentrations. In conclusion, the most effective antibiotics were Ciprofloxacin and Levofloxacin against the Pa.
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