PJB-2023-37
SCREENING AND REGENERATION OF CALLUS UNDER SELECTION PRESSURE OF NaCl
SARWAT NAZ
Abstract
The current study was done to exploit somaclonal variation for regeneration of salinity tolerant Brassica plantlets. The Viable calli of Brassica napus L. variety Hyola 401 formed on MS medium with 0.1mg/l 2,4-D were screened at higher level of NaCl (> 150mM). Regeneration of the survived calli was done on MS medium containing 2 mg/l BAP and 5 mg/l AgNO3. The multiple shoots formed were separated from callus and shifted to separate regeneration medium containing various levels of NaCl (0, 50, 100, 150, 200 and 250 mM). The screened multiple shootlets were then transferred to half strength MS medium augmented with various NaCl levels (0, 50, 100, 150, 200 and 250 mM) for rooting. The growth performances of regenerated plantlets were compared with germinating seedling of Hyola 401, mother plant (MP). Results indicated that Brassica napus L. Hyola 401 somaclone S9 was more tolerant as compared to mother plant (MP) and other regenerated somaclones. All the growth parameter (shoot length, root length, root/shoot ratio, shoot fresh weight, root fresh weight, shoot and root turgid weight, shoot dry weight, relative water content) showed significant differences among all varieties at different salt levels.
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