PJB-2023-33
AN EFFICIENT DETERMINATION OF PHENOLIC COMPOUNDS BY HPLC-DAD AND THEIR BIOACTIVITY ASSAY FROM AERIAL PARTS OF EUCALYPTUS TERETICORNIS
Abdul Waheed Chaudhary
Abstract
Current study is based on the extraction of free and bound phenolic compounds in aerial parts i.e. leave, stem, seed and skin of E. Tereticornis plant followed by using two different extraction procedures (Sonication and ultrasonic assisted base hydrolysis) and their antimicrobial activity against two bacteria i.e. E. coli and S. aureus was analyzed. The RP-HPLC-DAD was applied for separation and detection of phenolic compounds. HPLC profiling revealed the existence of 13 phenolic compounds in which 11 were phenolic acids and 2 aldehydes derivatives, while bound phenolic compounds possessing greater fraction than free ones. The results from ultrasonic base hydrolysis revealed the gallic (23.47 mg/g), p-coumaric (16.66 mg/g), p-hyroxybenzoic (11.03 mg/g) and ferulic (8.71 mg/g) acids while Sonication extraction method shows the chlorogenic (8.14 mg/g), vanillic (11.47 mg/g), sinapic (4.68 mg/g) and caffeic (5.38 mg/g) acids as main constituents in skin, seed, stem and leaves respectively. Four phenolic compounds were identified as bound phenolic acids i.e., p-coumaric, Cinnamic, naringenin and catechin, while rest of the compounds were found in both extracts. The free radical scavenging activity (RSA) and total Phenolic contents (TPC) were present in greater amount in seed (164.61 Mm.g–1) and skin (118.96 Mm/g) while total flavonoids and total tannins are higher in stem (42.77 Mm/g) and (28.13 Mm/g) respectively. E. Tereticornis leaves, stem, seed and skin extracts observed to be highly effective for Escherichia coli and Staphylococcus aureus. The antimicrobial action of E. Tereticornis extracts is higher against S. aureus (MIC value 125 μg/ml) than E. coli (MIC value 250 μg/ml).
To Cite this article:
Download
