PJB-2022-541
DEVELOPMENT AND CROSS-SPECIES TRANSFERABILITY OF EST-SSR MARKERS BASED ON DE NOVO TRANSCRIPTOMIC ASSEMBLY OF AESCULUS CHINENSIS BUNGE
YU LING LI
Abstract
Aesculus chinensis Bunge, a deciduous tree in the genus Aesculus of the family Hippocastanaceae, has ornamental, economic, and medicinal values. However, the lack of comprehensive genetic information hinders the utilization of this species, especially the EST-SSR markers in A. chinensis Bunge have not been reported previously. In this study, de novo assembly of A. chinensis Bunge transcriptomes was conducted, which enabled the identification of 33,365 unigenes with a mean length of 1348 bp. A total of 25450 (76.27%) unigenes showed similarities with at least one database including Nr (71.39%), Swiss Prot (57.03%), GO (53.31%), KO (27.13%) and KOG (19.27%). Based on the transcriptome data collected for A. chinensis, 16,103 SSR loci were detected with a distribution density of one SSR per 2.79 kb. The most dinucleotide and trinucleotide repeats were AG/CT and AAG/CTT. Then 58 primer pairs were randomly selected for polymorphism validation and 31 primer pairs were polymorphic among eight Aesculus species. A total of 167 alleles were identified, varying from 2 to 9 with a mean of 5.387 alleles per locus. According to the allelic variation,7 primers were capable of distinguishing the 8 Aesculus plants.
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