PJB-2022-501
The influence of various concentrations of essential oils on the growth rate of fibrolytic and amylolytic bacteria isolated from rumen fluid
Zeynep Sahan
Abstract
The antibacterial capabilities of essential oils (EOs) have been shown to influence the rumen microbial population. On the basis of their antimicrobial properties, six EOs were selected: oleaster, orange peel, laurel, garlic, thyme and cinnamon. Scanning Electron Microscopy pollen morphotypes were identified to reliably identify the plant species from which EOs are extracted. The maximum specific growth rate of bacteria is one of the metrics that are used to quantify the growth rate of bacteria. Dose–response incubations were carried out so that their effect could be determined on the maximum specific growth rate (µmax) of bacteria. Ten different concentrations (0, 50, 100, 200, 300, 400, 600, 800, 1000, and 5000 ppm) were used for this purpose. In order to measure bacterial growth, the optical density at 650 nm was measured hourly until the reading for bacterial growth decreased. Using Micro Fit, the maximum bacterial growth rate before growth began was calculated (v 1.0). It was determined that the influence of essential oils, dosages, and the relationship between dose and oil had a statistically significant impact on the maximal specific growth rate (p<0001). The EO of thyme was found to have the most potent antibacterial effect on all bacteria examined in the study. Following thyme EO, laurel, oleaster, and cinnamon EOs displayed the strongest antibacterial effect. Based on this study, it could be concluded that the antibacterial effect was generally more pronounced at 400 ppm doses of essantial oils
To Cite this article:
Sahan, Z. 2024. The influence of various concentrations of essential oils on the growth rate of fibrolytic and amylolytic bacteria isolated from rumen fluid. Pak. J. Bot., 56(1): DOI: http://dx.doi.org/10.30848/PJB2024-1(36)
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