PJB-2022-500
HIGH-DENSITY GENETIC MAP CONSTRUCTION AND QTL
YANLONG LIU
Abstract
Sorghum-sudangrass, a hybrid of sorghum and sudangrass that has the characteristics of high productivity, strong adaptability, and superior quality, is widely used in animal husbandry and aquaculture. Parsing the genetic basis of major agronomic traits is very important for sorghum and sudangrass hybrid breeding. In this study, we using a hybrid of sweet sorghum M81 and Sudangrass Sa constructe 174 F11 populations. Single-nucleotide polymorphisms (SNPs) derived from genotyping by Restriction-site Associated DNA Sequence (RAD-seq) of RILs were used to construct a high-density genetic map comprising 790 SNPs spanning 2101 cM and coverage of all 10 chromosomes was greater than 80%. Inclusive Composite Interval Mapping (ICIM) was carried out to map quantitative trait loci (QTL) for five forage traits. A total 18 QTLs were identified for plant height (PH, 4 QTLs), fresh weight (FW, 8 QTLs), dry weight (DW, 3 QTLs), stem diameter (SD, 2 QTLs), and sugar content (SC, 1 QTL). In addition, two overlapping QTLs (qFW9.2/qDW9 and qFW10/qDW10.2) were detected for FW and DW. Together, The RIL populations and high-density linkage maps in this study provide a theoretical basis for future sorghum-sudan grass hybrid forage breeding.
To Cite this article:
Download
