PJB-2022-490
Analysis of Antimicrobial Potential of Silver Nanoparticles Synthesized by Leaf Extract of Moringa oleifera
Iqra Mujahid
Abstract
In the present study silver nanoparticles synthesized from leaves extract of sohanjna (Moringa oleifera), were characterized and evaluated for antimicrobial effects. The characterization of silver nanoparticles was made by color change; UV-visible spectroscopy and Fourier transform infrared (FTIR) techniques. Antimicrobial potential silver nanoparticles was analyzed using disc diffusion method against clinically important Gram positive (Geobacillus, Staphylococcus aureus, Bacillus subtilis), Gram negative bacteria (Klebsiella pneumonia, Psuedomonas aeruginosa, Escherichia coli) and a fungal species, Candida albicans. The antimicrobial activity was assayed by size of growth inhibition zone developed by silver nanoparticles, and its dilutions 3:1, 1:1, in comparison to the inhibition activity exhibited by positive control (amoxicillin / fluconazole) and negative control (deionized water) against the tested strains of bacteria and fungus. The largest growth inhibition zone (17.00±0.577) was observed on staphylococcus aureus among Gram positive bacteria, while in Gram negative bacteria, the largest zone of inhibition (17.66±1.2) was obtained on pseudomonas aerginosa. The results revealed that there is insignificant difference among zone of inhibition developed by pure silver nanoparticles solution and its dilutions (17.00±0.577, 16.00±0.577 & 15.66±0.881). Moreover, there is insignificant difference between the zone of inhibition developed by positive control and nanoparticle solution (18.66±1.20 & 17.00±0.577). But the zone of inhibition on Candida albicans developed by nanoparticles is significantly small as compared to positive control (17.66±0.33 & 12.66±0.33). Taken together, our findings revealed that silver nanoparticles showed the most significant inhibition activity (p=0.00) against the tested strains of Gram positive, Gram negative bacteria but this activity is poor on Candida albicans as exhibited by inhibitory zone.
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