PJB-2022-423
ANTIMICROBIAL ACTIVITY OF PHENOLIC AND FLAVONOIDS ENRICHED EXTRACTS OF JURINEA DOLOMIAEA: A MEDICINAL PLANT FROM WESTERN KASHMIR HIMALAYAS
MUHAMMAD JAMIL AHMED
Abstract
The objective of current study was to investigate total phenolic, flavonoids and triterpenoids content in various solvent extracts from leaves and roots of Jurinea dolomiaea and their inhibitory effect against pathogenic bacteria and fungi strains. Disc diffusion method was adopted to find out antibacterial and antifungal potential of various solvent ectracts. Total phenolic, flavonoids and triterpenoids content were determined by Folin–Ciocalteu, aluminium chloride colorimetric method and vanillin–glacial acetic acid method respectively. Their correlation with antimicrobial activities were statistically analyzed by Pearson,s method. The present study revealed highest phenolic and flavonoids content in methanol and aqueous extracts in both leaves and roots of J. dolomiaea. The highest triterpenoid content was recorded in petroleum ether extract. Various solvents extracts from leaves and roots showed significant bactericidal effect against both Gram negative and Gram Positive bacteria. However, only aqueous and methanol extracts of Jurinea exhibited remarkable zone of inhibition against post-harvest fungal pathogens. Pearson’s correlation revealed a significant positive correlation between total phenolic and flavonoids content in various solvent extracts in both leaves and roots with their antibacterial activity. The findings from present study revealed that antibacterial activities of different solvent extracts from leaves and roots were due to highest phenolic and flavonoids content. Thus the study insight the presences of major bioactive compounds in plant extract and showed their potential antimicrobial activities against multi-drug resistant bacteria.
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