PJB-2022-418
Comprehensive study of the main biologically active compounds in Arctium lappa plants growing in Kazakhstan
Meruyert Abdikerim, Gulbayra Azimbaeva, Bakshat Izteleu and Kenzhegul Smailova
Abstract
For the first time, authors have studied biologically active compounds in Arctium lappa plants growing in Kazakhstan. The demand for biologically active substances is very high in today's global market. They are used in the food industry, in medicine, as well as in folk healing. The aim of the research is to study the total content of phytochemical components from growing Arctium lappa in Kazakhstan, located in the city of Almaty, Medeu district. Modern results of the quantitative content of pectin substances, phenolic acids, tannins, anthocyanins, flavonoids and other biologically active substances in Arctium lappa plants are presented. The macro-and microelement composition was determined by the atomic absorption method on a Shimadzu AA 7000 spectrometer, as a result of the studies, the elemental analysis of the plant showed the highest content of macronutrients, namely K and Ca, and of the trace elements Zn. The amino acid composition of the gas-liquid apparatus was also determined by the chromatographic method of the brand “Carlo-Erba-4200”. 20 amino acids were identified. Of these, glutamate (2485 mg/100 g), aspartate (1295 mg/100 g) and alanine (1008 mg/100 g). A method for the determination of fat and water-soluble vitamins by sequential analysis was developed. The method is based on capillary electrophoresis. Water-soluble polysaccharides, hemicellulose A and B were isolated from the underground part of the plant Arctium lappa. Their composition and structure were determined. The isolated hemicellulose A and B were recorded with a potassium bromide tablet in the infrared spectrum of the brand “Specord M-80” the zone 400-4000 cm-1. Phenolic compounds have been isolated from plants of Arctium lappa, including flavonoids pyrocatechin and polyphenols: ethylvaniline, quercetin, myricetin. The obtained compounds were identified based on the results of ultraviolet-visible, infrared, and proton nuclear magnetic resonance spectra.
To Cite this article:
Abdikerim, M., G. Azimbaeva, B. Izteleu and K. Smailova. 2024. Comprehensive study of the main biologically active compounds in Arctium lappa plants growing in Kazakhstan. Pak. J. Bot., 56(2): DOI: http://dx.doi.org/10.30848/PJB2024-2(8)
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