PJB-2022-403
Phytochemical analysis, DNA barcoding and DNA protective activity of Ferulago cassia Boiss.
Erkan Yilmaz, Miray Ege, Duygu Misirli, Gorkem Deniz Sonmez, Omer Kilic and Mahfuz Elmastas
Abstract
Chemical composition of the methanol extracts of Ferulago cassia Boiss. were identified by HPLC PDA and their DNA protective activity pattern determined. Regarding the chemotaxonomic evaluation, genetic analysis (barcoding) of species was carried out to contribute to differentiation of Ferulago species or phylogenetically close to other taxa. Chicoric acid and chlorogenic acid were found to be major compounds in both flowers and leaves extracts of F. cassia. Both methanol extracts were tested for DNA protective activity. Both of the extracts completely protected the pBluescript II SK (+) plasmid DNA from oxidative stress induced by UV and H2O2 in a DNA damage inhibition assay in which flower extract showed slightly more protection. The extract showed a great potential for DNA protection. ITS (internal transcribed spacer) sequence of F. cassia was obtained for the first time for DNA barcoding. The phylogenetic tree showed that F. cassia is closely related to F. macrocarpa
To Cite this article:
Yilmaz, E., M. Ege, D. Misirli, G.D. Sonmez, O. Kilic and M. Elmastas. 2023. Phytochemical analysis, DNA barcoding and DNA protective activity of Ferulago cassia Boiss. Pak. J. Bot., 55(6): DOI: http://dx.doi.org/10.30848/PJB2023-6(31)
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