PJB-2022-14
“GENETIC VARIATION AND BIOINFORMATICS ANALYSIS OF TEF2 GENE OF SORDARIA FIMICOLA"
Amna Rasheed
Abstract
The current study aimed to find out genetic variation and post-translational modifications of the eukaryotic translational elongation factor (eEF-1). Ribosomal translation factors are directly involved in protein translation and are greatly conserved but several studies have revealed that eEF1A is post-translationally modified. Six parental strains of Sordaria fimicola (S1, S2, S3, N5, N6, and N7) were subjected to amplify the TEF2 gene, and a comparison was made with reference species Saccharomyces cerevisiae. Different bioinformatics tools, i.e., Clustal W, PMes Server, PAIL and NetNES Servers, NetPhos 3.1, and Phyre2 were used for multiple sequence alignment, to identify methylation sites, acetylation sites on the protein, phosphorylation sites, and Protein 3D structure. The PTMs of S. fimicola strains were compared with PTMs of reference species. Out of the total, 329 nucleotides polymorphism was observed on five nucleotide positions. Altogether, a total of 13 sites (8Ser/4Thr/2Y) of phosphorylation modifications were observed in S. cerevisiae and S. fimicola. The polymorphism was observed at 23 sites in strains of SFS (S1, S2, and S3) while six polymorphic sites were present in NFS strains (NFS). The study reiterated that environmental stress causes genetic variation and ultimately leads to evolution.
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