PJB-2006-116
SOLUBLE PROTEINS INDUCED BY LOW TEMPERATURE TREATMENT IN THE LEAVES OF SPRING AND WINTER WHEAT CULTIVARS
G. KARIMZADEH*, G. R. SHARIFI-SIRCHI, M. JALALI-JAVARAN, H. DEHGHANI AND D. FRANCIS1
Abstract
Quantitative and qualitative changes in total leaf soluble proteins were determined in a spring cv. Zagross and in a winter cv. Azar 2 cultivar of allohexaploid wheat (Triticum aestivum L.) exposed to 4°C for two weeks. The data obtained were used to test whether these different genotypes of wheat responded differentially to cold treatment. Seedlings were grown in a controlled growth chamber for 14 d at 20°C and then transferred to 4°C for 14 d before returning them to 20°C (cold treatment), or else they were maintained continuously at 20°C (control treatment). The plants were sampled every 48 h for total leaf fresh weight measurements. Total leaf soluble proteins were extracted. Proteins concentration was either determined by a colorimetric method, or size-fractionated on SDS-PAGE. Clear cold-induced increases in proteins quantity occurred during the low temperature treatment irrespective of cultivar. However, the electrophoretic protein patterns showed differences between-cultivar and between-temperature treatment. Ten new cold-induced polypeptides (17
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