PJB-2021-1547
Identification and in-vitro assessment of a glycoprotein elicitor isolated from Colletotrichum falcatum: the causal agent of red rot in sugarcane
Ambreen Khadija Alvi and Javed Iqbal
Abstract
The fungal pathogen Colletotrichum falcatum, the causal agent of red rot disease, is one of the most damaging pathogens of sugarcane (Saccharum spp.). The identification of elicitors that initiate defense mechanism in plants against pathogen has become an area of considerable interest in the era where scientists are aiming to reduce the use of fungicides. In this study Colletotrichum falcatum spores were isolated from infected stalk pieces of sugarcane and grown on Czapek Dox agar medium to obtain pure culture. Then spores were obtained from pure culture and grown in Czapek Dox liquid medium for isolation of cell wall elicitor from two week old mycelial mat of Colletotrichum falcatum. Crude elicitor was purified by gel filtration using Sephadex G-200 column. Twenty three fractions were collected and analyzed for the presence of sugars and proteins for further identification of elicitor molecule. Sugarcane callus was prepared from explants of red rot resistant genotype HSF-240 on agar solidified MS basal medium augmented with 3-4 mg L-1 2,4-D (2,4-dichlorophenoxy acetic acid). Suspension cultured cells were prepared after formation of white friable callus which was used for further studies. The third fraction (F3) induced the highest PAL activity (2.181 m mol g-1 f. wt. h-1) and phenolic contents (0.196 mg ml-1) 6 h after treatment in sugarcane suspension-cultured cells of the resistant genotype, HSF-240, and was considered to have the active elicitor molecules. Moreover, this fraction itself had higher soluble proteins (0.999 mg ml-1) and sugars (1.87 mg ml-1) which confirmed that the elicitor molecules were glyco-protein in nature. The molecular weight of the elicitor molecule was calculated by drawing a regression plot from the regression equation (y = -66658x + 36) and estimated to be approximately 2133.446 kDa
To Cite this article:
Alvi, A.K. and J. Iqbal. 2021. Identification and in-vitro assessment of a glycoprotein elicitor isolated from Colletotrichum falcatum: the causal agent of red rot in sugarcane. Pak. J. Bot., 53(5): DOI: http://dx.doi.org/10.30848/PJB2021-5(45)
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