PJB-2006-109
MASS PROPAGATION OF BOUGAINVILLEA SPECTABILIS THROUGH SHOOT TIP CULTURE
SYED TARIQ SHAH, R. ZAMIR, T. MUHAMMAD AND *H. ALI
Abstract
A simple and efficient In vitro regeneration protocol for Bougainvillea spectabilis Willd., was developed from shoot tips of 5 year old plants. Shoot tips were surface sterilized and cultured on MS medium supplemented with different concentrations of BAP (0.25 - 2.0mg/l) or Kinetin (0.25 - 2.0mg/l) and NAA (0.1-0.5 mg/l) in combination with BAP (0.25 - 5.0 mg/l). It was observed that BAP (0.25mg/l) combined with 0.1mg/l NAA gave best results where 90% shoots were developed into plantlets. Best multiple shoot formation was recorded on MS medium supplemented with 1.0mg/l BAP and 250 mg/ l glutamine.
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