PJB-2006-64
PROPAGATION OF CTV-FREE SWEET ORANGE [CITRUS SINENSIS (L.) OSBECK] PLANTS THROUGH MICROBUDDING TECHNIQUE
MAZHAR ABBAS, M. M. KHAN,*, S. M. MUGHAL1, MUHAMMAD J. JASKANI1 AND HAIDER ABBAS2
Abstract
Microbudding technique was used for the early propagation of sweet orange [Citrus sinensis (L.) Osbeck] cv. Musambi plants, free of citrus tristeza virus (CTV) using thermotherapy. Source plants of sweet orange, both infected and apparently healthy, were collected from the orchards at Sahiwal and Faisalabad and analyzed along with microbudded plants through ELISA. Budwood of 3 mm and 4 mm in size was microbudded at two different heights (15 cm and 23 cm). Maximum (50%) success of microbudding was obtained, when budwood of 4mm was microbudded at the height of 23 cm. It would suggest that microbudding could efficiently be used for the early propagation of CTV free Musambi plants.
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