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Alkaline pre-treatment of wheat straw for production of ethanol using Saccharomyces cerevisiae SS-4 immobilized in nanoparticles
Abstract
This study was designed to evaluate cost effective bioprocess to enhance production of bioethanol by utilizing immobilized S. Cerevisiae SS-4 in calcium alginate (Ca) beads and calcium alginate magnetic nanoparticles (Ca-MNP) beads to improve bio ethanol production using wheat straw.The method of pre-treatment had a pronounced effect on the yield of reducing sugars.Maximum delignification (58%) and 36.31% saccharification was observed with 2.5% (v/v) NaOH. As concentration of NaOH increases, cellulose content decreases while increase in weight loss was observed. Results showed that immobilized cells of S. cerevisiae SS-4 in Ca-MNP gave high yield of ethanol (49.71g/L) as compared to immobilized cells of S. cerevisiae SS-4 in Ca (45.66 g/L) and free cells (36.52g/L) at pH 4.5, 28 ºC for 72 hrs. Magnet recovery method was used to recover nanoparticles and reusability was evaluated after five time usage
