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Pak. J. Bot., 48(4): 1421-1429, 2016.

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EVALUATION OF ADVANCED CHICKPEA (CICER ARIETINUM L.) ACCESSIONS BASED ON DROUGHT TOLERANCE INDICES AND SSR MARKERS

AGAINST DIFFERENT WATER TREATMENTS

MUHAMMAD AMIR MAQBOOL¹, MUHAMMAD ASLAM^1*,

HINA ALI² AND TARIQ MAHMUD SHAH²

¹Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

²Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan

^*Corresponding author’s email: aslampbg@gmail.com

Abstract

Chickpea is mainly grown on marginal lands and encounter the problem of erratic rainfall that causes lack of water availability especially at terminal growth stages. Forty advanced chickpea genotypes were grown under irrigated, rainfed and tunnel conditions for two years (2012-13 and 2013-14). Data were collected for seed yield and analyzed by analysis of variance. Highly significant differences among genotypes and water treatments were observed for seed yield. However, across the year differences were insignificant for seed yield of chickpea. Seed yield under rainfed was higher than under irrigated conditions. Forty genotypes were assembled in four distinct groups on the basis of PCA biplot for different drought tolerance indices. These four distinct groups were representative of genotypic performance under normal and stressed conditions. Twenty eight SSR primers were used for sortation of genotypes either as drought tolerant or susceptible and to find association with results of drought tolerance indices. Only nine SSR markers were found to be polymorphic while others were either monomorphic or not amplified. H3DO5 and TA8 with Group-I, TR19 and ICCM0035 with Group-II, ICCM0035 with Group-III and TA25 was strongly correlated with results of Group-IV. Genotypes of group-I were drought tolerant whereas, CH16/06, CH81/06 and D097-11 within this groups were more tolerant.

Key words: Water deficit, Pakistan, Irrigation, Tunnel, Rainfed, Marker assisted selection and PCA Biplot.