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Pak. J. Bot., 48(4):1385-1392, 2016

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IS DROUGHT TOLERANCE IN MAIZE (ZEA MAYSL.) CULTIVARS ATTHE JUVENILE STAGE MAINTAINEDAT THE REPRODUCTIVE STAGE?

NAHIDAH BASHIR¹, SEEMA MAHMOOD¹, ZAFAR ULLAH ZAFAR¹, SUMAIRA RASUL²,

HAMID MANZOOR² AND HABIB-UR-REHMAN ATHAR^1*

¹Institute of Pure and Applied Biology, BahauddinZakariya University, Multan60800, Pakistan

²Institute of Molecular Biology and Biotechnology, BahauddinZakariya University, Multan 60800, Pakistan

*Corresponding author's email: habibathar@yahoo.com

Abstract

Among several abiotic stresses, drought or water scarcity is a major constraint for crop production in many parts of the world. Six maize (Zea mays L.) cultivars; DTC, EV-77, EV-78, EV-79, Faisalabad mays, and 6621 were evaluated for drought tolerance at germination and seedling stages. Distilled deionized water was used as control but uniform drought stress was induced using 3, 6 and 9% of polyethylene glycol-6000 (PEG-6000) which correspond to osmotic potential of  -0.0466, -0.0759 and -0.0876 MPa, respectively. PEG influenced the germination and growth of the cultivars in a concentration dependent manner but the highest level of PEG induced more drastic decline for the various attributes studied. The cultivars showed significantly variable responses to different levels of PEG. The result of study clearly suggested variability of characters for drought tolerance among maize cultivars. Based on the pattern of variability for various attributes, 3 groups of cultivars can be classified. The cultivar 6621 had a consistent degree of sensitivity to drought in terms the reduction of various attributes studied. The second group includes DTC which showed a steady tolerance [(germination percentage (GP), energy of emergence (EG), germination rate (GR), root fresh and dry weight (RFW and RDW), shoot fresh and dry weight (SFW and SDW), dry biomass tolerance index (DBTI) and seedling vigor index (SVI)] thus seemed to provide some manifestation of drought tolerance. For the third group of cultivars, pattern of drought tolerance was independent for germination, growth and physiological indices as an incoherent variability of attributes was observed. A similar pattern of variability for a number of characters to simulated water stress in the cultivar DTC served as reliable determinants for drought tolerance in maize.  To assess maintenance of degree of drought tolerance selected maize cultivars, a field experiment was also conducted. Kernel yield, 1000- kernel weight (g), number of kernel number/cob, kernel weight/cob (g) was maximally reduced in water stress sensitive cv. 6621 whereas it was maximal in drought tolerant cv DTC. Drought stress at the reproductive stage hindered the floral development and/or fertilization process and thus yield reduction occurs. Overall, selection procedure for selecting drought tolerant maize cultivars was efficient at the germination and seedling growth stages.

Keywords: germination;maize(Zea mays L.); PEG; physiological indices; polyethylene glycol; water stress.