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Pak. J. Bot., 48(2): 681-688, 2016. |
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Updated: 01-04-16 | ||||
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GENETIC ANALYSIS OF VITIS INTERSPECIFIC HYBRIDS OCCURRING IN VINEYARDS OF THE CZECH REPUBLIC
RADDOVA JANA1*, STEFKOVA ANDREA, SOTOLAR RADEK2 AND MIROSLAV BARANEK1
Abstract: SSR analysis of 18 unknown uncultured Vitis genotypes planted in an area mostly dedicated to viticulture in the Czech Republic was performed in this work. The aim of this study was to identify analysed samples by comparing their SSR profiles with described standards and classify their mutual relationships based on their distribution in obtained dendrogram. Results show that 50% of unknown genotypes belongs to old American interspecific cultivar 'Noah' and 11% belong to another old American cultivar 'Isabella'. The rest of analysed genotypes remain unidentified, but three of them suggest relatedness with 'Noah' cultivar, one genotype shows relatednessto 'Isabella' cultivar. From practical point of view the most interesting ones are three genotypes, which were clearly clustered with the genotypes of cultural varities (botanically V. vinifera L.) used as standard. Based on this it is then possible to assume that those genotypes probably originated from crossing of non-V. vinifera genotype specimen with unknown cultural variety. Potential importance of analysed hybrids for further investigation and breeding, especialy in an “eco-friendly” viticulture, is also discussed.
Key words: Vitis, Interspecific hybrids, SSR, Identification.
Mendel University of Agriculture and Forestry in Brno, Faculty of Horticulture in Lednice 1The Mendeleum - Institute of Genetics and Plant Breeding, 691 44 Lednice, Czech Republic 2Department of viticulture and enology, 691 44 Lednice, Czech Republic *Corresponding author’s e-mail:radj@zf.mendelu.cz |
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