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GENOME-WIDE IDENTIFICATION, MOLECULAR CHARACTERIZATION AND EXPRESSION ANALYSIS OF THE ROP GTPase FAMILY IN PEPPER (CAPSICUM ANNUUM)

DINGQUAN HUANG¹, SHUILIN HE^2* AND MINGYANG LI^1*

Abstract: ROP/RAC GTPases is a plant-specific subfamily of Rho GTPases that plays a versatile role in the regulation of plant growth, development, in hormone signal transduction and response to the environment. Prior to the present study, only one Rop gene in pepper has been described. However, with the recent release of the draft genome sequence of pepper allowes us to conduct a genome wide search to identify how many Rop family members existed in pepper genome. We carried out bioinformatics analysis to establish the conserved as well as divergent regions on the protein sequences, phylogenetically analysis and the corresponding result shows that, CaROPs could be distributed into four groups as described in the literature for their homologs in Arabidopsis. To understand the function of nine Rop genes in pepper, we accordingly studied the tissue, fruit development and ripening expression patterns of CaRop genes by obtained RNA-seq data from public database. From our analysis, we realized that the expression of CaRop genes shows no total tissue or developmental specific expression. Furthermore, gene expression profiles of CaRop in response to environment stresses and hormone treatment, such as inoculated with Ralstonia solanacearum, by heat stress as well as treated with four phytohormones respectively and evaluated with real time RT-PCR. The potential involvement of specific CaRop genes in growth, fruit development, ripening, environment stresses as well as hormone responses discussed and may lay the foundation for future functional analysis to unravel their biological roles.

Key words: ROP GTPase, Pepper, Phylogenetic analysis, Phytohormones and Ralstonia solanacearum.

¹College of Horticulture and Landscape, Chongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, Southwest University, Beibei 400715, Chongqing, China

²College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China

* Corresponding author’s e-mail: shlhe201304@aliyun.com, limy@swu.edu.cn,