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Pak. J. Bot., 47(4): 1271-1280, 2015. |
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Updated: 18-08-15 | ||||
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A COMPARATIVE PROTEOMICS ANALYSIS OF PINUS MASSONIANA INOCULATED WITH BURSAPHELENCHUS XYLOPHILUS
ZHENG HUAYING1.2*, XU MING2, XU FUYUAN2 AND YE JIANREN１
Abstract: Pine wilt disease caused by Bursaphelenchus xylophilus, also known as pine wood nematode (PWN), is the most devastating disease of pine trees. Test results from different geographical provenance of P. massoniana inoculated with nematodes, indicated that GuangDong5 (GD5) has strong resistance to PWN. This article used GD5 as the experimental material, and sensitive SX1 as comparison. Total proteins were extracted and separated by 2-DE and MALDI-TOF-TOF MS technology from the pine needles of GD5 and SX1, respectively. Differentially expressed proteins in the provenance between inoculated and un-inoculated with PWN for two weeks, were analyzed. At last, 87 differentially expressed proteins were successfully identified by MALDI-TOF-TOF. The results also showed that there were six proteins involved in hydrogen peroxide scavenging capacity and protecting the redox homeostasis system from being damaged. Their up-regulation may be the main cause of the provenance GD5 resistant to PWN.
Key words: Pine wood nematode, Pine wilt, Proteomics, MALDI-TOF-TOF.
1College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037 1Forestry Academy of Jiangsu Province, Nanjing 211153, Dongshanqiao, China *Corresponding author’s e-mail: jbs_tech@163.com |
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