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Pak. J. Bot., 47(1): 197-202, 2015.

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ASSESSMENT OF GENETIC DIVERSITY AND VARIATION OF ACER MONO MAX SEEDLINGS AFTER SPACEFLIGHT

YUHAN SUN^1,2,§, YUYAO ZHANG^2,§, CUNQUAN YUAN^1,§, QING YANG³,  CUI LONG¹, YUN LI^1,* AND MINSHENG YANG^2,*

Abstract: Genetic diversity and variation of Acer Mono Maxim seedlings sampled from space-mutated (sm) populations were compared to seedlings from parallel control (ck) ones using molecular markers. RAMP analysis showed that the percentage of polymorphic band, Shannon diversity index and Nei’s gene diversity index of the space-mutated populations were higher than those of the control ones, which indicated that genetic variation increased after spaceflight in populations of Acer Mono Maxim. By using un-weighted pair group method with arithmetic mean (UPGMA) method, three space-mutated repeats (populations) were clustered together, and control groups clustered separately, which further indicated that there was difference between the space-mutated ones and the control ones, which may be caused by space mutation. Further analysis of genomic inconsistency between the root and leaf samples from the same tree showed that a total variation rate of 6.3% and 1.7% were obtained in ten space-mutated individuals by using RAMP and SSR markers, respectively, however, the variation rate was zero in control ones. It provided that space mutation may be caused the individual variation of Acer Mono Maxim.

Key words: Acer Mono Maxim; Genetic variation; Space mutation;

¹National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology,

Beijing Forestry University, Beijing 100083, China

²Forestry College, Agricultural University of Hebei, Baoding 071000, China

³School of Life Science, Beijing Normal University, Beijing 100083, China

^§Yuhan Sun, Yuyao Zhang, and Cunquan Yuan contributed equally to this study

^*Corresponding author’s e-mail: yunli@bjfu.edu.cn; deu100@yahoo.com.cn; Tel.: +86 10 62336094