|
Pak. J. Bot., 47(1): 159-170, 2015. |
Back to Contents | ||||
|
|
Updated: 24-02-15 | ||||
|
|
EFFECTS OF COLCHICINE TREATMENT ON THE MICROTUBULE CYTOSKELETON AND TOTAL PROTEIN DURING MICROSPOROGENESIS IN GINKGO BILOBA L.
YUHAN SUN1, YARU WANG1, 2, YINGYUE LI1, JINZHONG JIANG1, 3, NINA YANG1, CHEN NIU4 AND YUN LI1*
Abstract: The purpose of this study was to examine the effects of colchicine treatment on the microtubule cytoskeleton and the expression of proteins during microsporogenesis in G. biloba, as observed by immunofluorescence and 2-DE analysis in microsporangia treated with colchicine. The results showed the microtubule structures were affected by the colchicine in Ginkgo biloba, but the treatment effect of the colchicine had certain limitation in G. biloba. The percentage of microsporocytes whose microtubule structures were affected by the colchicine treatment was less than that observed in other plant species, not higher than 10 %. It was also found that the expression level of several endogenous proteins were changed in G. biloba when the microsporangia were treated with colchicine. Although we only tested colchicines was only tested in the present study, G. biloba appeared to possess factors that restricted the effect of such chemical agents. Our observations led us to speculate that the endogenous proteins are possibly responsible for the reduced effects of colchicine treatment in G. biloba.
1National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083; 2Shijiazhuang Pomology Institute, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, China 050061; 3Forest Plant Graduate School, Guizhou Normal College, Guiyang, China 550018; 4Driscoll Strawberry Associates, Inc. USA *Corresponding author’s e-mail: yunli63@gmail.com |
||||
|
|
|||||
| Back to Contents |
|
Back to Contents | |||
|
|
|||||