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Updated: 24-02-15 | ||||
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EFFECTS OF EXOGENOUS GROWTH REGULATORS ON CELL SUSPENSION CULTURE OF “YIN-HONG” GRAPE (VITIS VINIFERA L.) AND ESTABLISHMENT OF THE OPTIMUM MEDIUM
YU CHAO1, JIAO CAI FENG1, WU YUE YAN1*, YANG XIAO1, YANG YAN JUN1 AND JI JIN JUN2
Abstract: Callus induced by stem of "Yin-hong" grape (Vitis vinifera L.) was used as materials and B5 medium as basic medium. The major growth parameters of cell suspension cultures with various levels of 1-Naphthaleneacetic acid(NAA) and 6-Benzyl aminopurine(6-BA)were investigated to provide a basis for the optimum medium of suspension cell cultures of “Yin-hong” grape regarding cell number, packed cell volume (PCV), dry cell weight (DCW), cell viability, and morphology. All data were analysed by of two-way analysis of variance (ANOVA). Results showed that the treatment of 6-BA and NAA would effect the cell growth dynamics, probably causing logarithmic phase in advance at higher levels of 6-BA. Different concentration of 6-BA and NAA had significant effects on cells' number, PCV, DCW and viability (p<0.05), while no-significant effect was observed on the cells morphology. The optimum medium for suspension cell cultures of “Yin-hong” grape was identified asB5+1.5 mg/L6-BA+1.5 mg/LNAA+ 250 mg/L casein hydrolysate + 30 g/L sucrose. With the optimum medium, the maximum number of suspension cells after the logarithmic growth phase was 34.78 × 108 / mL, the highest cell viability reached 86.45%.; DCW reached 3.84 g/L and PCV reached 0.092 mL/mL after eight days cultivating.
Key words: "Yin-hong" grape, Cell suspension, Exogenous growth regulator, Optimum medium.
1College of Biological & Environmental Sciences, Zhejiang Wanli University, China 2NingboJieshun Biology Science & Technology Co., Ltd, China *Corresponding author e-mail: wuyueyan2013@163.com |
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