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CLONING AND
EXPRESSION ANALYSIS OF A NOVEL AMMONIUM TRANSPORTER GENE FROM EICHHORNIA
CRASSIPES
YUANMEI LI1, LIHUA JIANG1, GUOHUA YAN1, LIJUN ZHENG1, MINGHUI FU1* AND
JINGPING PENG2
Abstract: In order to explore the
molecular mechanism for Eichhornia crassipes to transport ammonium from
outside, we cloned a novel ammonium transporter (EcAMT) gene from E.
crassipes and identified its function by using yeast complementation
experiment. The full-length cDNA of EcAMT contains a 1506 nucletide-long
open reading frame which encodes a protein of 501 amino acids.
Bioinformatics analysis predicted that EcAMT had 8 transmembrane
regions. The expressions of EcAMT gene under three different nitrogen
conditions were evaluated by quantitative reverse transcriptase PCR (qRT-PCR)
and the results showed that the expression of EcAMT gene was
up-regulated under nitrogen starvation. Our study results revealed some
molecular mechanism of E. crassipes to absorb the ammonium in eutrophic
water.
Key words: Eichhornia crassipes;
Ammonium transporter; Clone; Expression.
1Department of Bioengineering, Guangdong
University of Technology, Guangzhou 510006, PR China
2Department of Chemistry Engineering, Guangdong University of
Technology, Guangzhou 510006, PR China
* Corresponding author. Tel.:+86 20 38457043; E-mail address:
mhfugd@126.com
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