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Pak. J. Bot., 46(5): 1735-1740, 2014.

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PRODUCTION OF ANTIHYPERTENSIVE PEPTIDES BY ENZYMATIC ZEIN HYDROLYSATE FROM MAIZE-ZEA MAYS SSP. MEXICANA INTROGRESSION LINE

LINGZHI WANG^1*, MINGLI QU², XIAOHUA ZHANG¹ AND YANJIANG QIAO^1*

Abstract: Teosintes are essential gene reservoir for maize breeding improvement, among which Zea mays ssp. mexicana has many valuable traits deserved to be transferred into maize genetic background. In this study, one maize-teosinte introgression line SD00100 was selected from the population of Zea mays ssp. mexicana as wild parent. This introgression line manifested the outstanding agricultural traits similar to maize parent Ye 515 and alien genetic material was identified by genomic in situ hybridization (GISH). To produce bioactive peptides with potent angiotensin converting enzyme (ACE) inhibitory activity, zein extracted from endosperm meal was then undergone enzymatic hydrolysis with thermolysin and the hydrolysate was then filtered through a 3 kDa cut-off membrane. ACE inhibitory activity of permeate from Ye 515 and SD00100 was evaluated by RP-HPLC. The IC₅₀ values of the peptides obtained from maize parent and the introgression line were 96.9 μg/ml and 22.9 μg/ml, respectively, with significant difference between them. Our results showed that an outstanding inbred maize line was obtained for production of antihypertensive peptides as well as for further development of functional food.

Keywords: Maize, Introgression line, Zein, Peptide, Hypertension

¹School of Chinese Materia Medica, Beijing University of Chinese Medicine, 100102, Beijing, China

²School of Life Science, Shandong University, 250100, Jinan, China

^*Corresponding author’s e-mail: yanyan30186@gmail.com; yjqiao@263.net