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Pak. J. Bot., 46(5): 1725-1729, 2014.

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ANTIOXIDANT POTENTIAL OF IMPATIENS BICOLOR ROYLE AND ZIZYPHUS OXYPHYLLA EDGEW

MUGHAL QAYUM¹, M. ZIA-Ul-Haq^2*, WAQAR A. KALEEM³, SHAKEEL AHMAD⁴, LUCA CALANI⁵, TERESA MAZZEO⁵, NICOLETTA PELLEGRINI⁵

Abstract: The present investigation has been carried out to evaluate the antioxidant capacity and phenolic composition of Impatiens bicolor Royle and Zizyphus oxyphylla Edgew. The content of phenolic compounds ranged from 15.77 to 27.61 mg catechin equivalents/g of different parts of Zizyphus oxyphylla Edgew., extract and 17.74 mg catechin equivalents/g for Impatiens bicolor Royle extract. The HPLC-ESI-MS/MS analysis of phenolic compounds showed that ferulic acid-hexosides was the only compound detected in I. bicolor, while Z. oxyphylla fruit, stem and leaves exhibited several compounds. Total antioxidant capacity values measured by TEAC assay were 46.32 ± 0.89, 42.56 ± 1.65, 41.34 ± 0.20, and 48.58 ± 0.21 µmol/g of extract, while those measured by FRAP assay were 102.40 ± 0.18, 207.54 ± 7.91, 254.89 ± 4.20, and 233.00 ± 9.07 µmol Fe²⁺/g, for I. bicolor and Z. oxyphylla fruit, leaves and stem, respectively. TRAP values were 43.26 ± 1.27, 112.23 ± 0.00, 102.83 ± 1.66, and 117.37 ± 3.70 µmol/g of extract for I. bicolor and Z. oxyphylla fruit, leaves and stem respectively. The results indicate that these two plants may be a potential source of antioxidants.

Key words: Impatiens bicolor Royle, Zizyphus oxyphylla Edew, Antioxidant activity.

¹Department of Pharmacy, Kohat University of Science & Technology, Kohat-26000, Pakistan

²The Patent Office, Karachi, Pakistan

³Department of Pharmacy, University of Swabi, Swabi-23430, Pakistan

⁴Department of Agronomy, Bahauddin Zakariya University, Multan-60800, Pakistan

⁵Department of Food Science, Human Nutrition Unit, University of Parma, Parco Area delle Scienze 59/A, IT-43124 Parma, Italy

^*Corresponding author e-mail: ahirzia@gmail.com