Pak. J. Bot., 46(3): 939-943, 2014.

ENHANCED PLANT REGENERATION IN LEMNA MINOR BY AMINO ACIDS

LIN YANG1, 2, HUAJUN HAN1, ZHAOJIANG ZUO1, KAIQIANG ZHOU1, CONG REN1, YERONG ZHU1, YANLING BAI1 AND YONGWANG1*

Abstract: In present study we investigated the effects of different L-amino acids on the plant regeneration from callus of Lemna minor, and established an efficient protocol. Among the 20 L-amino acids, only L-Ser and L-Gly showed significant improving effect, with the optimal concentration being 1 mM and 1.5 mM, respectively. A regeneration frequency of 46% was observed when the callus transferred to the regeneration medium with addition of 1 mM L-Ser for 11 days. After 26 days of cultivation, the frond regeneration achieved 100% and 94% for 1 mM L-Ser and 1.5 mM L-Gly treatment, respectively.

1College of Life Sciences, Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, China

2Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences,

Tianjin Normal University, 300387, Tianjin, China

*Corresponding author’s e-mail: wangyong@nankai.edu.cn; Tel: +862223504388; Fax: +862223508800