Pak. J. Bot., 46(3): 779-787, 2014. |
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Updated: 05-06-14 | ||||
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Genetic diversity analysis of Brassica napus/Brassica campestris progenies using microsatellite markers
Laila Fayyaz1, Farhatullah1*, M. Ashiq Rabbani2, Sidra Iqbal1 Mehwish Kanwal1 AND IFFAT NAWAZ1
Abstract: Genetic
diversity and relationship of F2 segregating progenies of
interspecific crosses between B. napus N-501/B. campestris
C-118 were studied. A set of 90 genotypes (2 parental lines and their
88 F2 progenies) was characterized separately using 24
microsatellite or SSR markers to cover the diversity as broadly as
possibly present in them. In initial screening only 12 out of 24 SSR
primers combination amplified DNA fragments, while the remaining 12
SSR primers did not amplify DNA fragment therefore those 12 SSR
molecular markers were not used for further analysis. The 12 SSR
primer combinations generated a total of 33 alleles, of that 32 were
polymorphic loci, whereas only one was monomorphic locus. Primers
BRMS-19 and BRMS-40 were highly polymorphic producing 4 bands each.
Primer Ra2-D04 was less polymorphic and it produced only one band. The
proportion of polymorphic loci was 95.83% which indicates high genetic
diversity among the progenies. The average number of polymorphic
alleles per locus was 2.66. The PIC values ranged from 0.395 for
primer Ra2-E03 to 0.726 for primer BRMS-019 with an average genetic
diversity (PIC value) of 0.584 per locus. Seven primers showed PIC
values above 0.5 (50%) indicating high genetic diversity in the
studied plant materials. Pair-wise similarity indices among 90
genotypes ranged from 0.3 to 0.95. Dendrogram obtained through UPGMA
clustering of F2 progenies depicted eight main groups using
similarity coefficient of 0.70. The progenies could be similar to
their parents if they have the same banding patterns as that of the
parents and could be distinguished from each other by the combination
of fragments which are repeatedly present in one progeny and absent in
the other. Considerable genetic diversity has been found among the F2
segregating progenies and their parents using SSR markers thus, SSR
analysis proved to be a useful tool. 1The University of Agriculture, Peshawar, Pakistan 2Plant Genetic Resources Institute, National Agricultural Research Centre, Islamabad, Pakistan *Corresponding author’s e-mail address: drfarhat@aup.edu.pk |
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