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MOLECULAR ANALYSES OF THE GENERA EREMOPYRUM (LEDEB.) JAUB. & SPACH AND AGROPYRON GAERTNER (POACEAE) BY PCR METHODS

REMZİYE YILMAZ¹, EVREN CABİ^2* AND MUSA DOGAN³

Abstract: RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) and Post PCR (Polymerase Chain Reaction) Melting Curve Analysis (MCA) have been used to investigate the pattern of genetic variation among some species in the genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae). Thirteen primers have been used in the study based on the RAPD-PCR and MCA analyses. Each species produced a distinct pattern of DNA fragments which have been used as a measure of the degree of relationship between species by means of using the RAPD-PCR results with three primers selected for identifying the genetic similarities. Polymorphic melting profiles have been obtained with Post PCR MCA method using three primers. Genetic similarities are calculated for all the species studied with RAPD-PCR and MCA methods, the dendrograms are obtained with the MVSP (Multi Variate Statistical Package) software using UPGMA (Unweighted Pair Group Method with Arithmetic Averages) and Jaccard’s Coefficient. Polymorphism between 18 populations of Eremopyrum and 6 Agropyron populations and within the species are determined by using RAPD-PCR and Post PCR melting curve analysis (MCA) respectively.

¹Middle East Technical University, Central Laboratory, Molecular Biology & Biotechnology R&D Center,

06530, Ankara, Turkey

²Namık Kemal University, Department of Biology, 59030 Tekirdağ, Turkey,

³Middle East Technical University, Department of Biological Sciences, 06530, Ankara-Turkey

*Corresponding author’s e-mail: ecabi@nku.edu.tr; ecabi2004@yahoo.com; Ph: +90-282-2502670