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Pak. J. Bot ., 45(6): 1905-1912, 2013. |
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Updated: 12-11-13 | ||||
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DIFFERENTIAL RESPONSES OF PHOTOSYSTEM II ACTIVITY TO PHOTOOXIDATION IN RED AND GREEN TISSUES OF AMARANTHUS TRICOLOR LEAVES
LING SHAO1,2ɸ, XIONGWEI CHEN1,2ɸ, YUANJUN CHEN1, BEIYU SUN1, WAHSOON CHOW3 AND CHANGLIAN PENG1*
Abstract: In order to study the antioxidative potential of amaranthine and its relationships with photoprotection, changes of PS II activity of red and green tissue in the same edible amaranth leaf were compared under photooxidation treatment induced by MV (methyl viologen) or H2O2. In the first 90 min of MV treatment, PS II maintained stable activity; the chlorophyll-fluorescence parameters were close to those of the controls. However, with the extension of treatment time, during 90-300 min of continuous photooxidative stress, Fv/Fm, Y (II) and Y (NPQ) of leaf discs decreased significantly, while Y (NO) and Fo dramatically increased, which showed that the activity of PS II suffered irreversible photooxidative damage, suppressed or even completely inhibited. Compared with the control, under 50 mmol·L-1 of H2O2 stress for 360 min, Y(NO) and Fo increased, accompanied by Y(NPQ) and the Fv/Fm decreases, while Y(II), qP and ETR were slightly increased, reflecting that treatment with 50 mmol·L-1 H2O2 promoted distribution of light energy in photochemistry reaction. After photooxidative treatments, a lower Fo and Y (NO) but higher Fv/Fm, Y (II), qP and Y (NPQ) was found in the red leaf discs compared with the green ones. The decrease and thedecline rate of the amaranthine content in the green and red leaf discs implied that the PS II of green leaf discs have a higher sensitivity to photooxidative damage than the red ones. It is suggested that the higher content of amaranthine may be used as a detoxification mechanism to counteract photooxidation in red leaf.1College of Life Science, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou 510631, China 2College of Life Science, Zhao Qing University, Zhaoqing 526061, China 3Division of Plant Science, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University, Canberra, Australian Capital Territory 0200, Australia *Corresponding author’s e-mail: pengchl@scib.ac.cn; Ph: +86-20-85217612; Fax: +86-20-85215535 ɸ These authors contributed equally to this work
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