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Updated: 30-05-13 | ||||
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CHROMOSOME LOCATION AND SSR MARKERS OF A STRIPE RUST RESISTANCE GENE FROM WHEAT (TRITICUM AESTIVUM) LINE N9738
BAOJU YANG1, WANQUAN JI1*, CHANGYOU WANG1*, FEI XUE1, M. ALI2, YAJUAN WANG1, HONG ZHANG1 AND XINLUN LIU1
Abstract: The objective of present study here was to determine the inheritance and chromosomal location of the resistance gene in N9738. The phenotypic data of F2 plants, from the crosses N9738/Huixianhong and N9738/Abbondanza, respectively, implied that the stripe rust resistance in N9738 was controlled by a single dominant gene, temporarily designated YrN9738. Monosomic (Nullisomic) analysis revealed YrN9738 is on wheat chromosome 1B. Seventy-eight SSR markers on chromosome 1B were screened on the parents and bulks. Four SSR markers (Xgpw7422, Xgpw5195, Xgpw7812, and Xcfd65) were used to construct a linkage map for YrN9738 and Xcfd65 was closely linked to YrN9738 with genetic distance of 3.5 cM. These linked markers were located on chromosome 1BL with Chinese Spring nullisomic-tetrasomics and ditelosomics of homoeologous group 1. Their presence in the deletion stocks of chromosome 1B located Xgpw7422 and Xgpw7812 in bin 1BL06-0.32-0.47 and Xgpw5195 in bin 1BL06-0.32. YrN9738 should be on wheat chromosome 1B near to the centromere and useful in wheat breeding programs.
1College of Agronomy, State Key laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, No. 3 Taicheng Road, 712100 Yangling, Shaanxi, China 2Department of Biotechnology, Sindh Agricultural University, Tandojam Sindh, Pakistan *Corresponding authors’s e-mail: jiwanquan2008@126.com; chywang2004@126.com; Phone: +86-29-87080200; +86-29-87082971 Fax: +86-29-87080200 |
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